KIFAP3 Rabbit Polyclonal Antibody
cat.: ER2001-69
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human KIFAP3 aa 1-200. |
| Positive control: | K562 cell lysate, rat testis tissue lysate, mouse hippocampus tissue lysates, rat bladder tissue, human liver carcinoma tissue, human thyroid tissue, SHSY5Y. |
| Subcellular location: | cytosol, endoplasmic reticulum, Golgi apparatus, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q92845 Human | P70188 Mouse | D3ZK15 Rat |
| Alternative names: | FLJ22818 KAP 3 KAP-3 KAP3 KIF3AP KIFA3_HUMAN Kifap3 Kinesin associated protein 3 Kinesin-associated protein 3 SMAP Smg GDS Smg GDS associated protein Smg GDS-associated protein |
Images
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Fig1:
Western blot analysis of KIFAP3 on different lysates with Rabbit anti-KIFAP3 antibody (ER2001-69) at 1/5,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-69, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 91.2kDa Observed band size: 91.2kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-KIFAP3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-69, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-KIFAP3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-69, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-KIFAP3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-69, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of KIFAP3 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.