Anti-Bit1 antibody
cat.: ER2001-71
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: Predicted band size: 13/19 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human Bit1 aa 1-179.
Positive control: PC-3M cell lysate, MCF-7 cell lysate.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB

1:500-1:1,000
Uniprot #: SwissProt: Q9Y3E5 Human
Alternative names: Bcl 2 inhibitor of transcription 1 Bcl-2 inhibitor of transcription 1 BIT 1 BIT1 CGI 147 CGI147 mitochondrial Peptidyl tRNA hydrolase 2 mitochondrial Peptidyl-tRNA hydrolase 2 PTH 2 PTH2 PTH2_HUMAN PTRH 2 PTRH2
Images
ER2001-71_1.jpg Fig1: Western blot analysis of Bit1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-3M cell lysate
Lane 2: MCF-7 cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.