Bit1 Rabbit Polyclonal Antibody
cat.: ER2001-71
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Bit1 aa 1-179. |
| Positive control: | Jurkat cell lysate, Raji cell lysate, MCF7 cell lysate, HepG2 cell lysate. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9Y3E5 Human |
| Alternative names: | Bcl 2 inhibitor of transcription 1 Bcl-2 inhibitor of transcription 1 BIT 1 BIT1 CGI 147 CGI147 mitochondrial Peptidyl tRNA hydrolase 2 mitochondrial Peptidyl-tRNA hydrolase 2 PTH 2 PTH2 PTH2_HUMAN PTRH 2 PTRH2 |
Images
|
Fig1:
Western blot analysis of Bit1 on different lysates with Rabbit anti-Bit1 antibody (ER2001-71) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: MCF7 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-71) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.