Bit1 Rabbit Polyclonal Antibody
cat.: ER2001-71
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 13/19 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Bit1 aa 1-179. |
| Positive control: | PC-3M cell lysate, MCF-7 cell lysate. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9Y3E5 Human |
| Alternative names: | Bcl 2 inhibitor of transcription 1 Bcl-2 inhibitor of transcription 1 BIT 1 BIT1 CGI 147 CGI147 mitochondrial Peptidyl tRNA hydrolase 2 mitochondrial Peptidyl-tRNA hydrolase 2 PTH 2 PTH2 PTH2_HUMAN PTRH 2 PTRH2 |
Images
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Fig1:
Western blot analysis of Bit1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3M cell lysate Lane 2: MCF-7 cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.