Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 63 kDa. |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human GRK1 aa 500-563. |
Positive control: | Mouse marrow tissue lysate, HUVEC cell lysate, 293 cell lysate, Rat marrow tissue lysate. |
Subcellular location: | Cell projection, Membrane. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: Q15835 Human | Q9WVL4 Mouse | Q63651 Rat |
Alternative names: | EC=2.7.11.14 G protein-coupled receptor kinase 1 GPRK1 Grk1 Rhodopsin kinase RHOK RK RK_HUMAN |
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Fig1:
Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse marrow tissue lysate Lane 2: HUVEC cell lysate Lane 3: 293 cell lysate Lane 4: Rat marrow tissue lysate |