GRK1 Rabbit Polyclonal Antibody
cat.: ER2001-72
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GRK1 aa 500-563. |
| Positive control: | Mouse marrow tissue lysate, HUVEC cell lysate, 293 cell lysate, rat marrow tissue lysate, mouse eyeball tissue, rat eyeball tissue, SHSY5Y. |
| Subcellular location: | Cell projection, Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q15835 Human | Q9WVL4 Mouse | Q63651 Rat |
| Alternative names: | EC=2.7.11.14 G protein-coupled receptor kinase 1 GPRK1 Grk1 Rhodopsin kinase RHOK RK RK_HUMAN |
Images
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Fig1:
Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse marrow tissue lysate Lane 2: HUVEC cell lysate Lane 3: 293 cell lysate Lane 4: Rat marrow tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue using anti-GRK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-72, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-GRK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-72, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of GRK1 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-72, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.