Cardiac Troponin T Rabbit Polyclonal Antibody
cat.: ER21102
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cardiac Troponin aa 11-60 / 298. |
| Positive control: | Mouse heart tissue lysate, rat heart tissue lysate, mouse heart tissue. |
| Subcellular location: | Cytosol |
| Recommended Dilutions:
WB IHC-P |
1:1,000-5,000 1:200 |
| Uniprot #: | SwissProt: P45379 Human | P50752 Mouse | P50753 Rat |
| Alternative names: | Cardiac muscle troponin T Cardiomyopathy dilated 1D (autosomal dominant) Cardiomyopathy hypertrophic 2 CMD1D CMH2 CMPD2 cTnT LVNC6 MGC3889 OTTHUMP00000033864 OTTHUMP00000033865 OTTHUMP00000033866 OTTHUMP00000033867 OTTHUMP00000033870 OTTHUMP00000218095 RCM3 TNNT 2 TNNT2 TNNT2_HUMAN TnTc Troponin T cardiac muscle Troponin T type 2 (cardiac) Troponin T type 2 cardiac Troponin T, cardiac muscle Troponin T2 cardiac |
Images
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Fig1:
Western blot analysis of Cardiac Troponin T on different lysates with Rabbit anti-Cardiac Troponin T antibody (ER21102) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER21102) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.