VCP Rabbit Polyclonal Antibody
cat.: ER30603
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 89 kDa
Isotype: IgG
Immunogen: Synthetic peptide within residues of VCP aa 757-806 / 806.
Positive control: A549 cell lysate, MCF7 cell lysate, HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, L929 cell lysate, F9 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, mouse heart tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat liver tissue lysate, rat kidney tissue lysate, Hela, HepG2, SHG-44, SW480, human uterus tissue, mouse skeletal muscle tissue, mouse heart tissue.
Subcellular location: Cytoplasm, nucleus, endoplasmic reticulum
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:2,000
1:200
1:200
Uniprot #: SwissProt: P55072 Human | Q01853 Mouse | P46462 Rat
Alternative names: 15S Mg(2+) ATPase p97 subunit 15S Mg(2+)-ATPase p97 subunit ALS14 ATPase p97 CDC48 IBMPFD MGC131997 MGC148092 MGC8560 p97 TER ATPase TERA TERA_HUMAN Transitional endoplasmic reticulum ATPase Valosin containing protein Valosin-containing protein VCP Yeast Cdc48p homolog
Images
ER30603_1.jpg Fig1: Western blot analysis of VCP on different lysates with Rabbit anti-VCP antibody (ER30603) at 1/2,000 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (10 µg/Lane)
Lane 3: HeLa cell lysate (10 µg/Lane)
Lane 4: Jurkat cell lysate (10 µg/Lane)
Lane 5: A431 cell lysate (10 µg/Lane)
Lane 6: L929 cell lysate (10 µg/Lane)
Lane 7: F9 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: Neuro-2a cell lysate (10 µg/Lane)
Lane 10: Mouse heart tissue lysate (20 µg/Lane)
Lane 11: Mouse brain tissue lysate (20 µg/Lane)
Lane 12: PC-12 cell lysate (10 µg/Lane)
Lane 13: Rat liver tissue lysate (20 µg/Lane)
Lane 14: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30603) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER30603_2.jpg Fig2: ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30603_3.jpg Fig3: ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30603_4.jpg Fig4: ICC staining VCP in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30603_5.jpg Fig5: ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30603_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.
ER30603_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.
ER30603_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.