VCP Rabbit Polyclonal Antibody
cat.: ER30603
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 89 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human VCP aa 757-806 / 806.
Positive control: A549 cell lysate, MCF7 cell lysate, HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, L929 cell lysate, F9 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, mouse heart tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat liver tissue lysate, rat kidney tissue lysate, HeLa, NIH/3T3, human uterus tissue, mouse skeletal muscle tissue, mouse heart tissue.
Subcellular location: Cytoplasm, nucleus, endoplasmic reticulum
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:2,000
1:250
1:200
Uniprot #: SwissProt: P55072 Human | Q01853 Mouse | P46462 Rat
Alternative names: 15S Mg(2+) ATPase p97 subunit 15S Mg(2+)-ATPase p97 subunit ALS14 ATPase p97 CDC48 IBMPFD MGC131997 MGC148092 MGC8560 p97 TER ATPase TERA TERA_HUMAN Transitional endoplasmic reticulum ATPase Valosin containing protein Valosin-containing protein VCP Yeast Cdc48p homolog
Images
ER30603_1.jpg Fig1: Western blot analysis of VCP on different lysates with Rabbit anti-VCP antibody (ER30603) at 1/2,000 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (10 µg/Lane)
Lane 3: HeLa cell lysate (10 µg/Lane)
Lane 4: Jurkat cell lysate (10 µg/Lane)
Lane 5: A431 cell lysate (10 µg/Lane)
Lane 6: L929 cell lysate (10 µg/Lane)
Lane 7: F9 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: Neuro-2a cell lysate (10 µg/Lane)
Lane 10: Mouse heart tissue lysate (20 µg/Lane)
Lane 11: Mouse brain tissue lysate (20 µg/Lane)
Lane 12: PC-12 cell lysate (10 µg/Lane)
Lane 13: Rat liver tissue lysate (20 µg/Lane)
Lane 14: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 89 kDa
Observed band size: 89 kDa

Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30603) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER30603_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling VCP with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER30603_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling VCP with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER30603_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.
ER30603_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.
ER30603_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.