VEGF Rabbit Polyclonal Antibody
cat.: ER30607
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 27 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human VEGFA.
Positive control: HeLa cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, MCF7 cell lysate, U-87 MG cell lysate, K-562 cell lysate, HepG2, HUVEC, human lung carcinoma tissue, Hela.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:5,000
1:200
1:200
1:100-1:200
Uniprot #: SwissProt: P15692 Human
Alternative names: Folliculostellate cell-derived growth factor Glioma-derived endothelial cell mitogen MGC70609 MVCD1 Vascular endothelial growth factor A vascular endothelial growth factor A121 vascular endothelial growth factor A165 vascular endothelial growth factor Vascular permeability factor VEGF A Vegf VEGF-A VEGF120 Vegfa VEGFA_HUMAN VPF
Images
ER30607_1.jpg Fig1: Western blot analysis of VEGF on different lysates with Rabbit anti-VEGF antibody (ER30607) at 1/5,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: HUVEC cell lysate
Lane 4: MCF7 cell lysate
Lane 5: U-87 MG cell lysate
Lane 6: K-562 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 39 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30607) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER30607_2.jpg Fig2: All lanes: Western blot analysis of VEGF with anti-VEGF antibody (ER30607) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2/3: VEGF knockdown Hela whole cell lysate (10 µg).

ER30607 was shown to specifically react with VEGF in wild-type Hela cells. Weakened bands were observed when VEGF knockdown samples were tested. Wild-type and VEGF knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER30607, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER30607_3.jpg Fig3: ICC staining VEGF in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30607_4.jpg Fig4: ICC staining VEGF in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30607_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.
ER30607_6.jpg Fig6: Flow cytometric analysis of Hela cells with VEGF antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.