Anti-Caspase-3 antibody
cat.: ER30804
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 31 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Caspase-3.
Positive control: Jurkat, F9, NCCIT, Hela, Raji, MCF-7, Daudi, A549, human tosil tissue, human liver cancer tissue, human lung tissue,human brain tissue,HepG2
Subcellular location: Nucleus, cytoplasm,
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:200
1:200
1:100-1:200
Uniprot #: SwissProt: P42574 Human
Alternative names: A830040C14Rik Apopain CASP 3 CASP-3 CASP3 CASP3_HUMAN Casp3a Caspase 3 Caspase 3, apoptosis-related cysteine peptidase Caspase 3, apoptosis-related cysteine protease Caspase 3, apoptosis-related cysteine protease a Caspase-3 subunit p12 Caspase3 CC3 CPP 32 CPP-32 CPP32 CPP32B Cysteine protease CPP32 EC 3.4.22.56 ICE3 LICE mldy OTTHUMP00000165052 OTTHUMP00000165053 OTTHUMP00000165054 PARP cleavage protease Procaspase3 Protein Yama SCA 1 SCA-1 SCA1 SREBP cleavage activity 1 Yama Yama protein
Images
ER30804_1.jpg Fig1: Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human lung
Lane 2: Human brain
Lane 3: Jurkat
Lane 4: F9
Lane 5: NCCTI
Lane 6: Daudi
Lane 7: Raji
Lane 8: MCF-7
Lane 9: Hela
ER30804_2.jpg Fig2: ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30804_3.jpg Fig3: ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30804_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
ER30804_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
ER30804_6.jpg Fig6: Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.