Caspase-3 Rabbit Polyclonal Antibody
cat.: ER30804
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC, IHC-Fr |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Caspase-3. |
| Positive control: | Jurkat cell lysate, Ramos cell lysate, human brain tissue lysate, HepG2, A549, human liver carcinoma tissue, human tonsil tissue. |
| Subcellular location: | Nucleus, cytoplasm, |
| Recommended Dilutions:
WB IF-Cell IHC-P FC IHC-Fr |
1:5,000 1:200 1:200 1:100-1:200 1:100 |
| Uniprot #: | SwissProt: P42574 Human |
| Alternative names: | Caspase3 A830040C14Rik Apopain CASP 3 CASP-3 CASP3 CASP3_HUMAN Casp3a Caspase 3 Caspase 3, apoptosis-related cysteine peptidase Caspase 3, apoptosis-related cysteine protease Caspase 3, apoptosis-related cysteine protease a Caspase-3 subunit p12 Caspase3 CC3 CPP 32 CPP-32 CPP32 CPP32B Cysteine protease CPP32 EC 3.4.22.56 ICE3 LICE mldy OTTHUMP00000165052 OTTHUMP00000165053 OTTHUMP00000165054 PARP cleavage protease Procaspase3 Protein Yama SCA 1 SCA-1 SCA1 SREBP cleavage activity 1 Yama Yama protein |
Images
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Fig1:
Western blot analysis of Caspase-3 on different lysates with Rabbit anti-Caspase-3 antibody (ER30804) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate Lane 3: Human brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30804) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
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Fig7:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Caspase-3 with Rabbit anti-Caspase-3 antibody (ER30804). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER30804, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig8:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Caspase-3 with Rabbit anti-Caspase-3 antibody (ER30804). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER30804, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.