IKK alpha Rabbit Polyclonal Antibody
cat.: ER30911
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Ikk alpha aa 1-50 / 745.
Positive control: Hela, NIH/3T3, HepG2, Raji, PC12, N2A, Jurkat, human mammary gland tissue, human gastric carcinoma tissue.
Subcellular location: Cytoplasm, nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:200
1:200
1:100-1:200
Uniprot #: SwissProt: O15111 Human
Alternative names: chuk CHUK1 Conserved Helix Loop Helix Ubiquitous Kinase Conserved helix loop ubiquitous kinase Conserved helix-loop-helix ubiquitous kinase I Kappa B Kinase 1 I Kappa B Kinase Alpha I-kappa-B kinase 1 I-kappa-B kinase alpha IkappaB kinase IkB kinase alpha subunit IkBKA IKK 1 IKK A IKK a kinase IKK-A IKK-alpha IKK1 IKKA IKKA_HUMAN Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit Inhibitor of nuclear factor kappa-B kinase subunit alpha NFKBIKA Nuclear Factor Kappa B Inhibitor Kinase Alpha Nuclear factor NF kappa B inhibitor kinase alpha Nuclear factor NF-kappa-B inhibitor kinase alpha Nuclear factor NFkappaB inhibitor kinase alpha Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor TCF-16 TCF16 Transcription factor 16
Images
ER30911_1.jpg Fig1: Western blot analysis of IKK alpha on different cell lysates using anti-IKK alpha antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Raji
Lane 4: NIH/3T3
Lane 5: PC12
Lane 6: Jurkat
ER30911_2.jpg Fig2: ICC staining IKK alpha in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER30911_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human mammary gland tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.
ER30911_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.
ER30911_5.jpg Fig5: Flow cytometric analysis of Hela cells with IKK alpha antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.