Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 85 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Ikk alpha aa 1-50 / 745. |
Positive control: | Hela, NIH/3T3, HepG2, Raji, PC12, N2A, Jurkat, human mammary gland tissue, human gastric carcinoma tissue. |
Subcellular location: | Cytoplasm, nucleus. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:200 1:100-1:200 |
Uniprot #: | SwissProt: O15111 Human |
Alternative names: | chuk CHUK1 Conserved Helix Loop Helix Ubiquitous Kinase Conserved helix loop ubiquitous kinase Conserved helix-loop-helix ubiquitous kinase I Kappa B Kinase 1 I Kappa B Kinase Alpha I-kappa-B kinase 1 I-kappa-B kinase alpha IkappaB kinase IkB kinase alpha subunit IkBKA IKK 1 IKK A IKK a kinase IKK-A IKK-alpha IKK1 IKKA IKKA_HUMAN Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit Inhibitor of nuclear factor kappa-B kinase subunit alpha NFKBIKA Nuclear Factor Kappa B Inhibitor Kinase Alpha Nuclear factor NF kappa B inhibitor kinase alpha Nuclear factor NF-kappa-B inhibitor kinase alpha Nuclear factor NFkappaB inhibitor kinase alpha Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor TCF-16 TCF16 Transcription factor 16 |
Fig1:
Western blot analysis of IKK alpha on different cell lysates using anti-IKK alpha antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Raji Lane 4: NIH/3T3 Lane 5: PC12 Lane 6: Jurkat |
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Fig2: ICC staining IKK alpha in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human mammary gland tissue using anti-IKK alpha antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-IKK alpha antibody. Counter stained with hematoxylin. |
Fig5: Flow cytometric analysis of Hela cells with IKK alpha antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |