Integrin beta 1 Rabbit Polyclonal Antibody
cat.: ER31001
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within residues of Integrin beta-1 aa 749-798 / 798. |
| Positive control: | Human liver, human kidney, NIH/3T3, A172, Hela, human liver carcinoma tissue, human colon carcinoma tissue, HepG2. |
| Subcellular location: | Cell membrane, cytoplasm |
| Recommended Dilutions:
WB FC IHC-P |
1:500-1:1,000 1:100 1:200 |
| Uniprot #: | SwissProt: P05556 Human | P09055 Mouse |
| Alternative names: | beta1 integrin CD29 Fibronectin receptor subunit beta FNRB Glycoprotein IIa GP IIa GPIIA Integrin beta-1 integrin VLA-4 beta subunit Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) ITB1_HUMAN ITGB1 MDF2 MSK12 OTTHUMP00000019420 Very late activation protein, beta polypeptide VLA BETA VLA-4 subunit beta VLA-BETA VLAB VLAbeta |
Images
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Fig1:
Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody (ER31001) at 1/1,000 dilution. Lane 1: A172 cell lysate Lane 2: Human kidney tissue lysate Lane 3: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 130 kDa Exposure time: 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31001) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Integrin beta 1 antibody (ER31001) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31001) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Integrin beta 1 antibody (ER31001) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31001) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of HepG2 cells with CD29 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.