Anti-CD133 antibody
cat.: ER31008
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 100 kDa
Isotype: IgG
Immunogen: Recombinant protein
Positive control: SW480, Hela, HepG2, HCT116, HT29, 293, HUVEC, SHG-44, human kidney tissue
Subcellular location: Cell membrane
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000
1:200
1:200
1:100-1:200
Uniprot #: O43490
Alternative names: AC133 antibody Antigen AC133 antibody CD133 antibody CORD12 antibody Hematopoietic stem cell antigen antibody hProminin antibody MCDR2 antibody MSTP061 antibody OTTHUMP00000217744 antibody OTTHUMP00000217745 antibody OTTHUMP00000217746 antibody PROM1 antibody PROM1_HUMAN antibody Prominin I antibody Prominin like 1 antibody Prominin like protein 1 precursor antibody Prominin mouse like 1 antibody Prominin-1 antibody Prominin-like protein 1 antibody Prominin1 antibody PROML1 antibody RP41 antibody STGD4 antibody
Images
ER31008_1.jpg Fig1: Western blot analysis of CD133 on different cell lysates using anti-CD133 antibody at 1/1000 dilution.
ER31008_2.jpg Fig2: ICC staining CD133 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31008_3.jpg Fig3: ICC staining CD133 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31008_4.jpg Fig4: ICC staining CD133 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31008_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.
ER31008_6.jpg Fig6: Flow cytometric analysis of SHG-44 cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
ER31008_7.jpg Fig7: Flow cytometric analysis of HUVEC cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.