GRB2 Rabbit Polyclonal Antibody
cat.: ER31203
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal residues of GRB2. |
| Positive control: | THP-1 cell lysate, MCF7 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, HepG2, human tonsil tissue, human breast cancer tissue, SHG-44. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P62993 Human | Q60631 Mouse | P62994 Rat |
| Alternative names: | Abundant SRC homology Adapter protein GRB2 ASH Ash protein EGFRBP GRB2 Epidermal growth factor receptor binding protein Epidermal growth factor receptor binding protein GRB2 GRB 2 GRB2 adapter protein Grb2 GRB2_HUMAN Grb3 3 Growth factor receptor bound protein 2 Growth factor receptor bound protein 3 Growth factor receptor-bound protein 2 HT027 MST084 MSTP084 NCKAP2 OTTHUMP00000166096 OTTHUMP00000166097 OTTHUMP00000166098 Protein ash SEM5 SH2/SH3 adapter GRB2 |
Images
|
Fig1:
Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (ER31203) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: F9 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 25/40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31203) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining GRB2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRB2 antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin. |
|
Fig5: Flow cytometric analysis of SHG-44 cells with GRB2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.