p70 S6 Kinase beta 1 Rabbit Polyclonal Antibody
cat.: ER31205
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to of Human p70 S6 Kinase beta aa 476-525 / 525. |
| Positive control: | PC12, Hela, NIH/3T3, Jurkat, HepG2, K562, Lovo, mouse pancreas tissue, rat pancreas tissue, human breast cancer tissue, human colon cancer tissue. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P23443 Human | Q8BSK8 Mouse | P67999 Rat |
| Alternative names: | 70 kDa ribosomal protein S6 kinase 1 KS6B1_HUMAN p70 alpha P70 beta 1 p70 ribosomal S6 kinase alpha p70 ribosomal S6 kinase beta 1 p70 S6 kinase alpha P70 S6 Kinase p70 S6 kinase, alpha 1 p70 S6 kinase, alpha 2 p70 S6K p70 S6K-alpha p70 S6KA p70(S6K) alpha p70(S6K)-alpha p70-alpha p70-S6K 1 p70-S6K P70S6K P70S6K1 p70S6Kb PS6K Ribosomal protein S6 kinase 70kDa polypeptide 1 Ribosomal protein S6 kinase beta 1 Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I RPS6KB1 S6K S6K-beta-1 S6K1 Serine/threonine kinase 14 alpha Serine/threonine-protein kinase 14A STK14A |
Images
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Fig1:
Western blot analysis of p70 S6 Kinase beta on different cell lysates using anti-p70 S6 Kinase beta antibody at 1/500 dilution. Positive control: Lane 1: PC12 Lane 2: NIH/3T3 Lane 3: Jurkat Lane 4: K562 Lane 5: Hela |
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Fig2: ICC staining p70 S6 Kinase beta in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining p70 S6 Kinase beta in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining p70 S6 Kinase beta in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of HepG2 cells with p70 S6 Kinase beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.