p70 S6 Kinase beta Rabbit Polyclonal Antibody
cat.: ER31205
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human p70 S6 Kinase beta aa 411-460. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, human pancreas tissue, HepG2. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:5,000 1:100-1:200 |
| Uniprot #: | SwissProt: P23443 Human | Q8BSK8 Mouse | P67999 Rat |
| Alternative names: | 70 kDa ribosomal protein S6 kinase 1 KS6B1_HUMAN p70 alpha P70 beta 1 p70 ribosomal S6 kinase alpha p70 ribosomal S6 kinase beta 1 p70 S6 kinase alpha P70 S6 Kinase p70 S6 kinase, alpha 1 p70 S6 kinase, alpha 2 p70 S6K p70 S6K-alpha p70 S6KA p70(S6K) alpha p70(S6K)-alpha p70-alpha p70-S6K 1 p70-S6K P70S6K P70S6K1 p70S6Kb PS6K Ribosomal protein S6 kinase 70kDa polypeptide 1 Ribosomal protein S6 kinase beta 1 Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I RPS6KB1 S6K S6K-beta-1 S6K1 Serine/threonine kinase 14 alpha Serine/threonine-protein kinase 14A STK14A |
Images
|
Fig1:
Western blot analysis of p70 S6 Kinase beta on different lysates with Rabbit anti-p70 S6 Kinase beta antibody (ER31205) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31205) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining p70 S6 Kinase beta in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-p70 S6 Kinase beta antibody (ER31205) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31205) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of HepG2 cells with p70 S6 Kinase beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.