MMP-1 Rabbit Polyclonal Antibody
cat.: ER31211
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MMP1 aa 432-475. |
| Positive control: | SK-Br-3 cell lysate, MCF7 cell lysate, Raji cell lysate, A431 cell lysate, HeLa cell lysate, HUVEC cell lysate, A549 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, human colon cancer tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P03956 Human | P81563 Rat |
| Alternative names: | 27 kDa interstitial collagenase CLG CLGN collagenase, fibroblast collagenase, interstitial Fibroblast collagenase Interstitial collagenase Matrix metallopeptidase 1 (interstitial collagenase) Matrix metalloprotease 1 Matrix Metalloproteinase 1 Matrix metalloproteinase-1 MMP 1 MMP-1 MMP1 MMP1_HUMAN OTTHUMP00000045866 |
Images
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Fig1:
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Raji cell lysate (15 µg/Lane) Lane 4: A431 cell lysate (15 µg/Lane) Lane 5: HeLa cell lysate (15 µg/Lane) Lane 6: HUVEC cell lysate (15 µg/Lane) Lane 7: A549 cell lysate lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lane 3: Raji cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.