Ubiquitin Rabbit Polyclonal Antibody
cat.: ER31212
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 8 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Ubiquitin aa 21-76.
Positive control: 293T cell lysate, HepG2 cell lysate, Hela cell lysate, MCF-7 cell lysate, Hela, HepG2, human tonsil tissue, mouse lung tissue, human kidney tissue.
Subcellular location: Cytoplasm, nucleus, Membrane, Mitochondrion, Mitochondrion outer membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500-1:1,000
1:200
1:200-1:1,000
Uniprot #: SwissProt: P0CG48 Human
Alternative names: Epididymis secretory protein Li 50 FLJ25987 HEL S 50 MGC8385 Polyubiquitin B RPS 27A RPS27A UBA 52 UBA 80 UBA52 UBA80 UBB UBB_HUMAN UBC UBCEP 1 UBCEP 2 UBCEP1 UBCEP2 Ubiquitin Ubiquitin B
Images
ER31212_1.jpg Fig1: Western blot analysis of Ubiquitin on different lysates with Rabbit anti-Ubiquitin antibody (ER31212) at 1/500 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: MCF-7 cell lysate treated with 50 µM MG132 for 1.5h

Lysates/proteins at 10 µg/Lane.

Predicted band size: 8 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31212) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER31212_2.jpg Fig2: Western blot analysis of Ubiquitin on different cell lysates using anti-Ubiquitin antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
ER31212_3.jpg Fig3: ICC staining Ubiquitin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31212_4.jpg Fig4: ICC staining Ubiquitin in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31212_5.jpg Fig5: ICC staining Ubiquitin in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31212_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Ubiquitin antibody (ER31212) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31212) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER31212_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ubiquitin antibody (ER31212) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31212) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.