CDk1 Rabbit Polyclonal Antibody
cat.: ER31213
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human CDk1. |
| Positive control: | MCF-7 cell lysate, Jurkat cell lysate, PC12 cell lysate, HepG2 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, SKBR3 cell lysate, Hela, HepG2, MCF-7, rat spleen tissue, human tonsil tissue, human breast cancer tissue, mouse spleen tissue, Hela. |
| Subcellular location: | Cytoplasm, nucleus, Cytoskeleton, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P06493 Human |
| Alternative names: | Cdc 2 Cdc2 CDC28A CDK 1 CDK1 CDK1_HUMAN CDKN1 CELL CYCLE CONTROLLER CDC2 Cell division control protein 2 Cell division control protein 2 homolog Cell division cycle 2 G1 to S and G2 to M Cell division protein kinase 1 Cell Divsion Cycle 2 Protein Cyclin Dependent Kinase 1 Cyclin-dependent kinase 1 DKFZp686L20222 MGC111195 p34 Cdk1 p34 protein kinase P34CDC2 |
Images
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Fig1:
Western blot analysis of CDk1 on different lysates using anti-CDk1 antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 3: PC12 cell lysate Lane 4: HepG2 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse liver tissue lysate Lane 8: SKBR3 cell lysate |
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Fig2: ICC staining CDk1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CDk1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CDk1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDk1 antibody. Counter stained with hematoxylin |
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Fig7: Immunohistochemical analysis of paraffin-e.mbedded human breast cancer tissue using anti-CDk1 antibody. Counter stained with hematoxylin |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of Hela cells with CDk1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.