CDk1 Rabbit Polyclonal Antibody
cat.: ER31213
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human CDk1. |
| Positive control: | MCF-7 cell lysate, Jurkat cell lysate, PC12 cell lysate, HepG2 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, SKBR3 cell lysate, HeLa, NIH/3T3, rat spleen tissue, human tonsil tissue, human breast cancer tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm, nucleus, Cytoskeleton, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: P06493 Human |
| Alternative names: | Cdc 2 Cdc2 CDC28A CDK 1 CDK1 CDK1_HUMAN CDKN1 CELL CYCLE CONTROLLER CDC2 Cell division control protein 2 Cell division control protein 2 homolog Cell division cycle 2 G1 to S and G2 to M Cell division protein kinase 1 Cell Divsion Cycle 2 Protein Cyclin Dependent Kinase 1 Cyclin-dependent kinase 1 DKFZp686L20222 MGC111195 p34 Cdk1 p34 protein kinase P34CDC2 |
Images
|
Fig1:
Western blot analysis of CDk1 on different lysates using anti-CDk1 antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 3: PC12 cell lysate Lane 4: HepG2 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse liver tissue lysate Lane 8: SKBR3 cell lysate |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling CDk1 with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling CDk1 with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDk1 antibody. Counter stained with hematoxylin |
|
Fig6: Immunohistochemical analysis of paraffin-e.mbedded human breast cancer tissue using anti-CDk1 antibody. Counter stained with hematoxylin |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin. |
|
Fig8:
Flow cytometric analysis of HeLa cells labeling CDk1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER31213, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.