PUMA Rabbit Polyclonal Antibody
cat.: ER31215
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal residues of PUMA. |
| Positive control: | K-562 cell lysate, HCT 116 cell lysate, SKOV-3, Hela, Lovo, human breast cancer tissue, mouse small intestine tissue, Jurkat. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:200 1:100 |
| Uniprot #: | SwissProt: Q9BXH1 Human |
| Alternative names: | BBC 3 Bbc3 BBC3_HUMAN BCL 2 binding component 3 Bcl-2-binding component 3 BCL2 binding component 3 JFY 1 JFY-1 JFY1 p53 up regulated modulator of apoptosis p53 up-regulated modulator of apoptosis p53 Upregulated Modulator of Apoptosis PUMA alpha PUMA/JFY1 |
Images
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Fig1:
Western blot analysis of PUMA on different lysates with Rabbit anti-PUMA antibody (ER31215) at 1/1,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: HCT 116 (Human colon cancer cell) cell lysate Lane 2: RAW264.7 treated with 200ng/ml mIFN-γ for 24 hours and 50ng/ml LPS for 24hours, and then treated with 300ng/ml BFA for 16 hours cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER31215, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20.5 kDa Observed band size: 21/23 kDa |
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Fig2: ICC staining PUMA in SKOV-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PUMA in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PUMA in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.