ER31216

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, ICC, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1 mg/mL.

Purification:

Peptide affinity purified

Molecular weight:

27 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within N-terminal human HSP27.

Positive control:

Hela, Jurkat, 293, mouse kidney tissue, human kidney tissue, mouse heart tissue,A172,Lovo,human colon cancer tissue, human tonsil tissue.

Subcellular location:

Cytoplasm, nucleus

Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500
1:100
1:200

Uniprot #:

P04792

Alternative names:

Heat shock 27kDa protein antibody 28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody epididymis secretory protein Li 102 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody heat shock protein family B (small) member 1 antibody HEL-S-102 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody HSP 27 antibody Hsp 28 antibody Hsp B1 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 27 antibody

	Fig1: Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: 293 Lane 4: Human kidney Lane 5: Mouse kidney Lane 6: Mouse heart
	Fig2: ICC staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Hsp27 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin.