Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, ICC, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Peptide affinity purified |
Molecular weight: | 27 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within N-terminal human HSP27. |
Positive control: | Hela, Jurkat, 293, mouse kidney tissue, human kidney tissue, mouse heart tissue,A172,Lovo,human colon cancer tissue, human tonsil tissue. |
Subcellular location: | Cytoplasm, nucleus |
Recommended Dilutions:
WB ICC IHC-P |
1:500 1:100 1:200 |
Uniprot #: | P04792 |
Alternative names: | Heat shock 27kDa protein antibody 28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody epididymis secretory protein Li 102 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody heat shock protein family B (small) member 1 antibody HEL-S-102 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody HSP 27 antibody Hsp 28 antibody Hsp B1 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 27 antibody |
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Fig1:
Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: 293 Lane 4: Human kidney Lane 5: Mouse kidney Lane 6: Mouse heart |
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Fig2: ICC staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Hsp27 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. |