Anti-HSP27 antibody
cat.: ER31216
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 27 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human HSP27.
Positive control: Hela, Jurkat, 293, mouse kidney tissue, human kidney tissue, mouse heart tissue,A172,Lovo,human colon cancer tissue, human tonsil tissue.
Subcellular location: Cytoplasm, nucleus
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500
1:100
1:200
Uniprot #: P04792
Alternative names: Heat shock 27kDa protein antibody 28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody epididymis secretory protein Li 102 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody heat shock protein family B (small) member 1 antibody HEL-S-102 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody HSP 27 antibody Hsp 28 antibody Hsp B1 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 27 antibody
Images
ER31216_1.jpg Fig1: Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: 293
Lane 4: Human kidney
Lane 5: Mouse kidney
Lane 6: Mouse heart
ER31216_2.jpg Fig2: ICC staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31216_3.jpg Fig3: ICC staining Hsp27 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER31216_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin.
ER31216_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Hsp27 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.