ER31216

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 23 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within N-terminal human HSP27.

Positive control:

Hela cell lysate, Jurkat cell lysate, 293 cell lysate, human kidney tissue lysate, A172, Lovo, human colon cancer tissue, human tonsil tissue.

Subcellular location:

Cytoplasm, nucleus, Cytoskeleton.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:100
1:200

Uniprot #:

SwissProt: P04792 Human

Alternative names:

Heat shock 27kDa protein 28 kDa heat shock protein CMT2F DKFZp586P1322 epididymis secretory protein Li 102 Estrogen regulated 24 kDa protein Estrogen-regulated 24 kDa protein Heat shock 25kDa protein 1 Heat shock 27 kDa protein Heat shock 27kD protein 1 Heat shock 27kDa protein 1 Heat shock 28kDa protein 1 Heat Shock Protein 27 Heat shock protein beta 1 Heat shock protein beta-1 heat shock protein family B (small) member 1 HEL-S-102 HMN2B HS.76067 Hsp 25 HSP 27 Hsp 28 Hsp B1 Hsp25 HSP27 Hsp28 HspB1 HSPB1_HUMAN SRP27 Stress responsive protein 27 Stress-responsive protein 27

	Fig1: Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293 cell lysate Lane 4: Human kidney tissue lysate
	Fig2: ICC staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Hsp27 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin.