CD31 Rabbit Polyclonal Antibody
cat.: ER31219
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal residues of CD31. |
| Positive control: | THP-1 cell lysate, Hela, HUVEC, NIH/3T3, SW480, Jurkat, human kidney tissue |
| Subcellular location: | Cell membrane, Cell junction. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P16284 Human | Q08481 Mouse |
| Alternative names: | Adhesion molecule CD31 CD31 antigen CD31 EndoCAM EndoCAM FLJ34100 FLJ58394 GPIIA GPIIA' PECA1 PECA1_HUMAN Pecam 1 PECAM 1 CD31 EndoCAM PECAM PECAM-1 Pecam1 Platelet and endothelial cell adhesion molecule 1 Platelet endothelial cell adhesion molecule Platelet/endothelial cell adhesion molecule 1 |
Images
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Fig1:
Western blot analysis of CD31 on THP-1 cell lysates with Rabbit anti-CD31 antibody (ER31219) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31219) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining CD31 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD31 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CD31 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining CD31 in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
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Fig8:
"Western blot analysis of CD31 on mouse lung tissue lysates with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31219) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature." |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.