ER31221

STAT1 alpha/beta Rabbit Polyclonal Antibody

cat.: ER31221

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 87/83 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Stat-1 alpha/beta 76-119
Positive control:	Jurkat cell lysate, A431 cell lysate, SK-Br-3 cell lysate, RAW264.7 cell lysate, L6 cell lysate, PC-12 cell lysate, HepG2, MCF-7, Hela, human colon tissue, human spleen tissue, mouse colon tissue, mouse spleen tissue, rat colon tissue, rat spleen tissue.
Subcellular location:	Cytoplasm, nucleus.
Recommended Dilutions: WB IF-Cell IHC-P	1:20,000 1:200 1:1,000
Uniprot #:	SwissProt: P42224 Human \| P42225 Mouse Entrez Gene: 25124 Rat
Alternative names:	CANDF7 DKFZp686B04100 ISGF 3 ISGF3 OTTHUMP00000163552 OTTHUMP00000165046 OTTHUMP00000165047 OTTHUMP00000205845 Signal transducer and activator of transcription 1 Signal transducer and activator of transcription 1, 91kDa Signal transducer and activator of transcription 1-alpha/beta Stat1 STAT1_HUMAN STAT91 Transcription factor ISGF-3 components p91/p84

Images

	Fig1: Western blot analysis of STAT1 alpha/beta on different lysates with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/20,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: L6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 87/83 kDa Observed band size: 87/83 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31221) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Stat-1α/β in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig4: ICC staining Stat-1α/β in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31221) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31221) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31221) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig8: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31221) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig9: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31221) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig10: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER31221) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.