Stat-1 alpha/beta Rabbit Polyclonal Antibody
cat.: ER31221
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Stat-1 alpha/beta 76-119 |
| Positive control: | MCF-7 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, HepG2, MCF-7, Hela, human breast cancer tissue, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P42224 Human |
| Alternative names: | CANDF7 DKFZp686B04100 ISGF 3 ISGF3 OTTHUMP00000163552 OTTHUMP00000165046 OTTHUMP00000165047 OTTHUMP00000205845 Signal transducer and activator of transcription 1 Signal transducer and activator of transcription 1, 91kDa Signal transducer and activator of transcription 1-alpha/beta Stat1 STAT1_HUMAN STAT91 Transcription factor ISGF-3 components p91/p84 |
Images
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Fig1:
Western blot analysis of Stat-1α/β on different cell lysates using anti-Stat-1α/β antibody at 1/500 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: MCF-7 cell lysate |
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Fig2: ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Stat-1α/β in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Stat-1α/β in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Stat-1α/β antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Hela cells with Stat-1α/β antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.