CDK6 Rabbit Polyclonal Antibody
cat.: ER40101
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within residues of CDK6 aa 267-326 / 326. |
| Positive control: | HeLa cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, K562 cell lysate, COS-1 cell lysate, Hela, HepG2, human lung cancer tissue, mouse thymus tissue, rat thymus tissue. |
| Subcellular location: | Cytoplasm, nucleus, Cell projection, Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:200 1:200 |
| Uniprot #: | SwissProt: Q00534 Human | Q64261 Mouse Entrez Gene: 114483 Rat |
| Alternative names: | CDK 6 CDK6 CDK6_HUMAN Cell division protein kinase 6 Crk 2 Crk2 Cyclin dependent kinase 6 Cyclin-dependent kinase 6 MGC59692 p40 PLSTIRE Serine/threonine protein kinase PLSTIRE Serine/threonine-protein kinase PLSTIRE STQTL11 |
Images
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Fig1:
Western blot analysis of CDK6 on different lysates with Rabbit anti-CDK6 antibody (ER40101) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40101) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2,000 dilution. Positive control: Lane 1: K562 cell lysate Lane 2: COS-1 cell lysate |
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Fig3: ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-CDK6 antibody (ER40101) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-CDK6 antibody (ER40101) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-CDK6 antibody (ER40101) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.