Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Green monkey, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 37 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within residues of CDK6 aa 267-326 / 326. |
Positive control: | Hela, HepG2, COS-1 cell lysate, K562 cell lysate, human colon cancer tissue, human breast cancer tissue. |
Subcellular location: | Cytoplasm, nucleus, Cell projection, Cytoskeleton. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:200 1:200 |
Uniprot #: | SwissProt: Q00534 Human |
Alternative names: | CDK 6 CDK6 CDK6_HUMAN Cell division protein kinase 6 Crk 2 Crk2 Cyclin dependent kinase 6 Cyclin-dependent kinase 6 MGC59692 p40 PLSTIRE Serine/threonine protein kinase PLSTIRE Serine/threonine-protein kinase PLSTIRE STQTL11 |
Fig1:
Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2,000 dilution. Positive control: Lane 1: K562 cell lysate Lane 2: COS-1 cell lysate |
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Fig2: ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDK6 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK6 antibody. Counter stained with hematoxylin. |