CDK6 Rabbit Polyclonal Antibody
cat.: ER40101
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Green monkey, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Synthetic peptide within residues of CDK6 aa 267-326 / 326.
Positive control: Hela, HepG2, COS-1 cell lysate, K562 cell lysate, human colon cancer tissue, human breast cancer tissue.
Subcellular location: Cytoplasm, nucleus, Cell projection, Cytoskeleton.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:2,000
1:200
1:200
Uniprot #: SwissProt: Q00534 Human
Alternative names: CDK 6 CDK6 CDK6_HUMAN Cell division protein kinase 6 Crk 2 Crk2 Cyclin dependent kinase 6 Cyclin-dependent kinase 6 MGC59692 p40 PLSTIRE Serine/threonine protein kinase PLSTIRE Serine/threonine-protein kinase PLSTIRE STQTL11
Images
ER40101_1.jpg Fig1: Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2,000 dilution.
Positive control:
Lane 1: K562 cell lysate
Lane 2: COS-1 cell lysate
ER40101_2.jpg Fig2: ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40101_3.jpg Fig3: ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40101_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDK6 antibody. Counter stained with hematoxylin.
ER40101_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK6 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.