MGMT Rabbit Polyclonal Antibody
cat.: ER40104
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within residues of MGMT aa 158-207 / 207. |
| Positive control: | MCF7 (Human breast cancer cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, MOLT-4 (Human acute T-Cell leukemia cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, A172, SK-BR-3, MCF-7, human tonsil tissue, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:200 1:200 1:100 |
| Uniprot #: | SwissProt: P16455 Human |
| Alternative names: | 6 O methylguanine DNA methyltransferase 6-O-methylguanine-DNA methyltransferase Agat AGT AI267024 EC 2.1.1.63 Methylated DNA protein cysteine methyltransferase Methylated-DNA--protein-cysteine methyltransferase Methylguanine DNA methyltransferase MGC107020 MGMT MGMT_HUMAN O 6 methylguanine DNA alkyltransferase O 6 methylguanine DNA methyltransferase O-6-methylguanine-DNA methyltransferase O-6-methylguanine-DNA-alkyltransferase |
Images
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Fig1:
Western blot analysis of MGMT on different lysates with Rabbit anti-MGMT antibody (ER40104) at 1/5,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: MDA-MB-231 (Human breast cancer cell) cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 4: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 6: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lane 7: Daudi (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Negative expression of MGMT protein in MDA-MB-231 is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER40104, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 22 kDa |
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Fig2:
Western blot analysis of MGMT on different lysates with Rabbit anti-MGMT antibody (ER40104) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MGMT KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40104) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MGMT antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.