Desmin Rabbit Polyclonal Antibody
cat.: ER40113
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Desmin aa 405-470/470. |
| Positive control: | Human heart tissue lysate, human skeletal muscle tissue lysate, mouse heart tissue lysate, human uterus tissue, mouse skeletal muscle tissue, mouse heart tissue, hybrid fish (crucian-carp) brain tissue lysate, HepG2, NIH-3T3, Hela. |
| Subcellular location: | Cytoplasm, Cell membrane, Intermediate filament, Membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:200 1:200 |
| Uniprot #: | SwissProt: P17661 Human |
| Alternative names: | CMD1I CSM1 CSM2 DES DESM_HUMAN Desmin FLJ12025 FLJ39719 FLJ41013 FLJ41793 Intermediate filament protein OTTHUMP00000064865 |
Images
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Fig1: ICC staining of Desmin in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining of Desmin in NIH-3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining of Desmin in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Desmin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Desmin antibody. Counter stained with hematoxylin. |
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Fig7:
Western blot analysis of Desmin on different lysates with Rabbit anti-Desmin antibody (ER40113) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER40113, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 54 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.