Ras Rabbit Polyclonal Antibody
cat.: ER40115
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ras aa 11-60 / 189. |
| Positive control: | PC12 cell lysate, MCF-7 cell lysate, 293T cell lysate, Mouse brain tissue lysate, Mouse intestine tissue lysate, Rat brain tissue lysate, HCT116, Hela, HepG2, MCF-7, mouse kidney tissue, rat small intestine tissue, human liver cancer tissue, human kidney muscle tissue, mouse kidney tissue, mouse small intestine tissue . |
| Subcellular location: | Cytoplasmic side, cell membrane, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P01111 | P01112 | P01116 |
| Alternative names: | C-BAS/HAS c-H-ras C-HA-RAS1 CTLO GTPase HRas GTPase KRas GTPase NRas H-Ras-1 H-RASIDX Ha-Ras HAMSV HRAS HRAS1 K RAS2A K RAS2B K RAS4A K RAS4B K-RAS KRAS KRAS1 KRAS2 N-RAS N-terminally processed NRAS NRAS1 p21ras RASH_HUMAN RASH1 RASK2 Transforming protein p21 v Ha ras Harvey rat sarcoma viral oncogene homolog v Ki ras2 Kirsten rat sarcoma viral oncogene homolog v ras neuroblastoma RAS viral oncogene homolog |
Images
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Fig1:
Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: PC12 cell lysate Lane 2: MCF-7 cell lysate Lane 3: 293T cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse intestine tissue lysate Lane 6: Rat brain tissue lysate |
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Fig2: ICC staining Ras in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Ras in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Ras in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining Ras in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ras antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded human kidney muscle tissue using anti-Ras antibody. Counter stained with hematoxylin. |
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Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin. |
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Fig11: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.