STAT6 Rabbit Polyclonal Antibody
cat.: ER40121
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 94 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within STAT6 aa 814-847/847. |
| Positive control: | Jurkat cell lysate, Raji cell lysate, Daudi cell lysate, Hela cell lysate, Ags cell lysate, human tosnsil tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P42226 Human |
| Alternative names: | 12S1644 D12S1644 IL 4 STAT IL-4 Stat IL4 STAT Interleukin 4 Induced Interleukin 4 Induced Transcription Factor IL4 STAT Signal transducer and activator of transcription 6 Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 Signal transducer and activator of transcription 6, interleukin 4 induced STAT 6 STAT interleukin4 induced STAT, interleukin4 induced Stat6 STAT6_HUMAN STAT6B STAT6C Transcription factor IL 4 STAT |
Images
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Fig1:
Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lane 3: Hela cell lysate Lane 4: Ags cell lysate Lane 5: Jurkat cell lysate |
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Fig2:
Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ER40121) at 1/500 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si STAT6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 94 kDa Observed band size: 94 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. ER40121 was shown to specifically react with STAT6 in Hela-si NT cells. Weakened band was observed when Hela-si STAT6 sample was tested. Hela-si NT and Hela-si STAT6 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER40121, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining STAT6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT6 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.