ROCK1 Rabbit Polyclonal Antibody
cat.: ER40122
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 158 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within residues of ROCK1 aa 1,305-1,354 / 1,354. |
| Positive control: | A431 cell lysate, Human lung tissue lysate, Mouse spleen tissue lysate, C6 cell lysate, L6 cell lysate, HAP1 cell lysate, human colon cancer tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, cell membrane, Nucleus, Cell projection, Cytoskeleton, Golgi apparatus, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:200 |
| Uniprot #: | SwissProt: Q13464 Human | P70335 Mouse | Q63644 Rat |
| Alternative names: | coiled-coil-containing protein kinase 1 coiled-coil-containing protein kinase I MGC131603 MGC43611 p160 Rhoassociated coiled coil-forming protein kinase p160 ROCK-1 p160 ROCK1 p160ROCK PRO0435 Renal carcinoma antigen NY REN 35 Renal carcinoma antigen NY-REN-35 Rho associated coiled coil containing protein kinase 1 Rho associated protein kinase 1 Rho kinase Rho-alpha kinase Rho-associated Rho-associated protein kinase 1 ROCK I ROCK-I ROCK1 ROCK1_HUMAN Rok rokalpha |
Images
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Fig1:
Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: C6 cell lysate Lane 5: L6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 158 kDa Observed band size: 158 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ROCK1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 158 kDa Observed band size: 158 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ROCK1 antibody (ER40122) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ROCK1 antibody (ER40122) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.