ER40122

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 158 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within residues of ROCK1 aa 1,305-1,354 / 1,354.

Positive control:

Hela cell lysate, NIH/3T3 cell lysate, Jurkat cell lysate, human colon cancer tissue, HepG2.

Subcellular location:

Cytoplasm, cell membrane, Nucleus, Cell projection, Cytoskeleton, Golgi apparatus, Membrane.

Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC

1:1,000
1:200
1:50
1:100-1:200

Uniprot #:

SwissProt: Q13464 Human

Alternative names:

coiled-coil-containing protein kinase 1 coiled-coil-containing protein kinase I MGC131603 MGC43611 p160 Rhoassociated coiled coil-forming protein kinase p160 ROCK-1 p160 ROCK1 p160ROCK PRO0435 Renal carcinoma antigen NY REN 35 Renal carcinoma antigen NY-REN-35 Rho associated coiled coil containing protein kinase 1 Rho associated protein kinase 1 Rho kinase Rho-alpha kinase Rho-associated Rho-associated protein kinase 1 ROCK I ROCK-I ROCK1 ROCK1_HUMAN Rok rokalpha

	Fig1: Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK1 antibody. Counter stained with hematoxylin.
	Fig3: Immunocytochemistry analysis of HepG2 cells labeling ROCK1 with Rabbit anti-ROCK1 antibody (ER40122) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ROCK1 antibody (ER40122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L(FITC) was used as the secondary antibody. Nuclear DNA was labelled in blue with DAPI.