Topoisomerase II alpha Rabbit Polyclonal Antibody
cat.: ER40124
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 174 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal residues of Topoisomerase II alpha. |
| Positive control: | Jurkat cell lysate, NIH/3T3 cell lysate, A431 cell lysate, L929 cell lysate, A549 cell lysate, Human liver tissue lysate, HUVEC cell lysate, A549, Hela, A431, NIH/3T3, human tonsil tissue, human colon cancer tissue, human colon cancer tissue, Hela. |
| Subcellular location: | Cytoplasm, nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P11388 Human |
| Alternative names: | alpha isozyme ATP hydrolyzing DNA topoisomerase II alfa DNA gyrase DNA topoisomerase (ATP hydrolyzing) DNA topoisomerase 2 alpha DNA topoisomerase 2-alpha DNA topoisomerase II 170 kD DNA topoisomerase II alpha isozyme DNA topoisomerase II DNA Topoisomerase2 TOP 2A TOP2 TOP2A TOP2A_HUMAN Topoisomerase DNA II alpha 170kDa TP2A |
Images
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Fig1:
Western blot analysis of TOP2A on different lysates using anti-TOP2A antibody at 1/500 dilution. Positive control: Lane 1: Jurkat cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: A431 cell lysate Lane 4: L929 cell lysate Lane 5: A549 cell lysate Lane 6: Human liver tissue lysate Lane 7: HUVEC cell lysate |
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Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin. |
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Fig9:
Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.