Anti-Topoisomerase II alpha antibody
cat.: ER40124
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 174 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal residues of Topoisomerase II alpha.
Positive control: A549, Hela, NIH/3T3, Jurkat, L929, A431, HUVEC, human liver tissue, human tonsil tissue, human breast cancer tissue, human colon cancer tissue.
Subcellular location: Cytoplasm, nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:200
1:200
1:100-1:200
Uniprot #: P11388
Alternative names: alpha isozyme antibody ATP hydrolyzing DNA topoisomerase II alfa antibody DNA gyrase antibody DNA topoisomerase (ATP hydrolyzing) antibody DNA topoisomerase 2 alpha antibody DNA topoisomerase 2-alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA topoisomerase II antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TOP2A_HUMAN antibody Topoisomerase DNA II alpha 170kDa antibody TP2A antibody
Images
ER40124_1.jpg Fig1: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3
Lane 3: A431
Lane 4: L929
Lane 5: A549
Lane 6: Human liver
Lane 7: HUVEC
ER40124_2.jpg Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40124_3.jpg Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40124_4.jpg Fig4: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40124_5.jpg Fig5: ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40124_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
ER40124_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
ER40124_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
ER40124_9.jpg Fig9: Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.