Catalase Rabbit Polyclonal Antibody
cat.: ER40125
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Catalase. |
| Positive control: | F9 cell lysate, A549 cell lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Human lung tissue lysate, Hela cell lysate, human liver tissue, rat kidney tissue, rat liver tissue, rat lung tissue, mouse liver tissue, mouse kidney tissue. |
| Subcellular location: | Peroxisome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P04040 Human | P24270 Mouse | P04762 Rat |
| Alternative names: | Cas1 CAT CATA_HUMAN Catalase Cs1 MGC138422 MGC138424 |
Images
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Fig1:
Western blot analysis of Caveolin-1 on different lysates using anti-Caveolin-1 antibody at 1/500 dilution. Positive control: Lane 1: F9 cell lysate Lane 2: A549 cell lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse liver tissue lysate Lane 5: Human lung tissue lysate Lane 6: Hela cell lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Catalase antibody (ER40125) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40125) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-catalase antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-catalase antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-catalase antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-catalase antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-catalase antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.