Anti-PDI antibody
cat.: ER40404
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal residues of PDI.
Positive control: Hela, MCF-7, PANC,HepG2, HUVEC, NIH/3T3, L929, F9, Jurkat, A431, A549, mouse liver tissue, mouse kidney tissue, mouse brain tissue,human lung carcinoma tissue,human kidney,Human brain,Human liver,human colon carcinoma tissue.
Subcellular location: Endoplasmic reticulum lumen
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:1,000
1:200
1:200
Uniprot #: Q13087
Alternative names: Pancreas specific protein disulfide isomerase antibody Pancreas-specific protein disulfide isomerase antibody Pancreatic protein disulfide isomerase antibody PDA2 antibody PDI antibody PDIA2 antibody PDIA2_HUMAN antibody PDIp antibody PDIR antibody Protein disulfide isomerase A2 antibody Protein disulfide isomerase antibody Protein disulfide isomerase associated 2 antibody Protein disulfide isomerase family A member 2 antibody Protein disulfide isomerase pancreatic antibody Protein disulfide-isomerase A2 antibody Rho GDP dissociation inhibitor gamma antibody
Images
ER40404_1.jpg Fig1: Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela     Lane 2: MCF-7
Lane 3: PANC     Lane 4: HepG2
Lane 5: HUVEC     Lane 6: NIH/3T3
Lane 7: L929     Lane 8: F9
Lane 9: Jurkat     Lane 10: A431
Lane 11: A549     Lane 12: Mouse liver
Lane 13: Mouse kidney   Lane 14: Mouse brain
Lane 15: Human kidney   Lane 16: Human brain
Lane 17: Human liver
ER40404_2.jpg Fig2: ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40404_3.jpg Fig3: ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40404_4.jpg Fig4: ICC staining PDI in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER40404_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
ER40404_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.