PDI Rabbit Polyclonal Antibody
cat.: ER40404
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within residues of PDI aa 476-525 / 525. |
| Positive control: | HEK-293 cell lysate, HT-29 cell lysate, NIH/3T3 cell lysate, HUVEC, A549, NIH/3T3, human lung carcinoma tissue, human colon carcinoma tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: Q13087 Human |
| Alternative names: | Pancreas specific protein disulfide isomerase Pancreas-specific protein disulfide isomerase Pancreatic protein disulfide isomerase PDA2 PDI PDIA2 PDIA2_HUMAN PDIp PDIR Protein disulfide isomerase A2 Protein disulfide isomerase Protein disulfide isomerase associated 2 Protein disulfide isomerase family A member 2 Protein disulfide isomerase pancreatic Protein disulfide-isomerase A2 Rho GDP dissociation inhibitor gamma |
Images
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Fig1:
Western blot analysis of PDI on different lysates with Rabbit anti-PDI antibody (ER40404) at 1/1,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; ECL: K1803 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER40404, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58.2 kDa Observed band size: 57 kDa |
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Fig2: ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PDI in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.