Chromogranin A Rabbit Polyclonal Antibody
cat.: ER40724
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Tissue, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to C-terminal of Mouse Chromogranin A aa 408-457 / 457 (ID: P10645). |
| Positive control: | PC12 cell lysate, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue, human colon cancer tissue, Lovo. |
| Subcellular location: | Cytoplasmic vesicle, secreted. |
| Recommended Dilutions:
WB IHC-P FC IF-Tissue IF-Cell |
1:2,000 1:200-1:1,000 1:100-1:200 1:200 1:100 |
| Uniprot #: | SwissProt: P10645 Human | P26339 Mouse | P10354 Rat |
| Alternative names: | CISY_HUMAN Citrate synthase Citrate synthase, mitochondrial citrate synthetase Cs EC 2.3.3 EC 2.3.3.1 |
Images
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Fig1: Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2,000 dilution. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ER40724) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER40724, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ER40724) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER40724, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Chromogranin A antibody (ER40724) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40724) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-chromogranin A antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Lovo cells with chromogranin A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
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Fig8:
Western blot analysis of Chromogranin A on different lysates with Rabbit anti-Chromogranin A antibody (ER40724) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 1: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50-75 kDa Observed band size: 50-75 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40724) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig9:
Immunocytochemistry analysis of PC-12 cells labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ER40724) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Chromogranin A antibody (ER40724) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.