MuERVL-Gag Rabbit Polyclonal Antibody
cat.: ER50102
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IF-Cell, WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse MuERVL-Gag aa 541-581/581. |
| Positive control: | Mouse embryo, ES-D3 cell lysate, ES-W4 cell lysate. |
| Recommended Dilutions:
IF-Cell WB |
1:200 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: V9H130 Mouse |
Images
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Fig1: ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4℃ with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). |
|
Fig2:
Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (ER50102) at 1/1,000 dilution. Lane 1: ES-D3 cell lysate Lane 2: ES-W4 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER50102) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.