HSP70 Rabbit Polyclonal Antibody
cat.: ER50802
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cow |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminalhuman HSP70. |
| Positive control: | A549, MCF-7, HCT116, A431, SHG-44, SKBR-3, mouse testis tissue, mouse prostate tissue, mouse brain tissue, human breast cancer tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:200 1:200 |
| Uniprot #: | SwissProt: P0DMV8 Human |
| Alternative names: | DnaK type molecular chaperone HSP70 1 Epididymis secretory protein Li 103 FLJ54303 FLJ54370 FLJ54392 FLJ54408 FLJ75127 Heat shock 70 kDa protein 1 Heat shock 70 kDa protein 1/2 Heat shock 70 kDa protein 1A/1B Heat shock 70kDa protein 1A Heat shock 70kDa protein 1B Heat shock induced protein HEL S 103 HSP70 1 HSP70 1B HSP70 2 HSP70-1/HSP70-2 HSP70-1A HSP70.1 HSP70.1/HSP70.2 HSP70I HSP71_HUMAN HSP72 HSPA1 HSPA1A HSPA1B |
Images
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Fig1:
Western blot analysis of HSP70 on different cell lysates using anti-HSP70 antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: MCF-7 Lane 3: HCT116 |
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Fig2: ICC staining HSP70 in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining HSP70 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining HSP70 in SKBR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP70 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP70 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP70 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-HSP70 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.