VEGF Receptor 1 Rabbit Polyclonal Antibody
cat.: ER50803
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 151 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human VEGFR1. |
| Positive control: | Mouse liver tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, human liver tissue, human kidney tissue. |
| Subcellular location: | Cell membrane, endosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P17948 Human |
| Alternative names: | EC 2.7.10.1 FLT 1 FLT Flt-1 FLT1 Fms like tyrosine kinase 1 Fms related tyrosine kinase 1 Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor Fms-like tyrosine kinase 1 FRT Soluble VEGF receptor 1 14 Soluble VEGFR1 variant 2 Soluble VEGFR1 variant 21 Tyrosine protein kinase FRT Tyrosine protein kinase receptor FLT Tyrosine-protein kinase FRT Tyrosine-protein kinase receptor FLT Vascular endothelial growth factor receptor 1 Vascular endothelial growth factor vascular permeability factor receptor Vascular permeability factor receptor 1 Vascular permeability factor receptor VEGFR 1 VEGFR-1 VEGFR1 VGFR1_HUMAN |
Images
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Fig1: Western blot analysis of VEGFR1 on mouse liver tissue lysate using anti- VEGFR1 antibody at 1/1000 dilution. |
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Fig2:
Western blot analysis of VEGFR1 on different lysates with Rabbit anti-VEGFR1 antibody (ER50803) at 1/1,000 dilution. Lane 1: MCF7-si NT cell lysate Lane 2: MCF7-si VEGFR1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 151 kDa Observed band size: 151 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. ER50803 was shown to specifically react with VEGFR1 in Hela-si NT cells. Weakened band was observed when Hela-si VEGFR1 sample was tested. Hela-si NT and Hela-si VEGFR1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER50803, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin. |
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Fig7:
Western blot analysis of VEGF Receptor 1 on different lysates with Rabbit anti-VEGF Receptor 1 antibody (ER50803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 151 kDa Observed band size: 151 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER50803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.