CAB39 Rabbit Polyclonal Antibody
cat.: ER60546
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CAB39 aa 1-341. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000-1:10,000 |
| Uniprot #: | SwissProt: Q9Y376 Human | Q06138 Mouse Entrez Gene: 301574 Rat |
| Alternative names: | Cab39 CAB39_HUMAN Calcium binding protein 39 Calcium-binding protein 39 CGI 66 CGI-66 CGI66 MO25 alpha MO25 Mouse protein 25 alpha Protein Mo25 |
Images
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Fig1:
Western blot analysis of CAB39 on different lysates with Rabbit anti-CAB39 antibody (ER60546) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: HCT 116 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER60546) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CAB39 on different lysates with Rabbit anti-CAB39 antibody (ER60546) at 1/10,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER60546) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.