ELL3 Rabbit Polyclonal Antibody
cat.: ER61160
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse ELL3 aa 251-397.
Positive control: Raji cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:10,000
Uniprot #: SwissProt: Q9HB65 Human | Q80VR2 Mouse | Q5XFX8 Rat
Alternative names: Elongation Factor RNA Polymerase II-Like 3 RNA polymerase II elongation factor ELL3 ELL3
Images
ER61160_1.jpg Fig1: Western blot analysis of ELL3 on different lysates with Rabbit anti-ELL3 antibody (ER61160) at 1/10,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 45 kDa
Observed band size: 40 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER61160) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.