Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 45 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within mouse ELL3 aa 251-397. |
Positive control: | Raji cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB |
1:10,000 |
Uniprot #: | SwissProt: Q9HB65 Human | Q80VR2 Mouse | Q5XFX8 Rat |
Alternative names: | Elongation Factor RNA Polymerase II-Like 3 RNA polymerase II elongation factor ELL3 ELL3 |
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Fig1:
Western blot analysis of ELL3 on different lysates with Rabbit anti-ELL3 antibody (ER61160) at 1/10,000 dilution. Lane 1: Raji cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER61160) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |