| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse ELL3 aa 251-397. |
| Positive control: | Raji cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: Q9HB65 Human | Q80VR2 Mouse | Q5XFX8 Rat |
| Alternative names: | Elongation Factor RNA Polymerase II-Like 3 RNA polymerase II elongation factor ELL3 ELL3 |
|
Fig1:
Western blot analysis of ELL3 on different lysates with Rabbit anti-ELL3 antibody (ER61160) at 1/10,000 dilution. Lane 1: Raji cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER61160) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |