ER61893

Stanniocalcin 1 / STC Rabbit Polyclonal Antibody

cat.: ER61893

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 28 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Stanniocalcin 1 aa 181-230.
Positive control:	SK-OV-3 cell lysate, U-87 MG cell lysate, Mouse brain tissue lysate, Mouse small intestine tissue lysate, Mouse pancreas tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate, U-87 MG, human kidney tissue, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue.
Subcellular location:	Secreted.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:5,000 1:50 1:200-1:1,000 1:1,000
Uniprot #:	SwissProt: P52823 Human \| O55183 Mouse \| P97574 Rat
Alternative names:	Stanniocalcin 1 Stanniocalcin Stanniocalcin-1 STC STC-1 Stc1 STC1_HUMAN

Images

Fig1: Western blot analysis of Stanniocalcin 1 / STC on different lysates with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/5,000 dilution.

Lane 1: SK-OV-3 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse small intestine tissue lysate
Lane 5: Mouse pancreas tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat colon tissue lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER61893) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of U-87 MG cells labeling Stanniocalcin 1 / STC with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

	Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER61893) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER61893) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER61893) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Stanniocalcin 1 / STC antibody (ER61893) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER61893) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of U-87 MG cells labeling Stanniocalcin 1 / STC.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER61893, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.