non-muscle Myosin IIA Rabbit Polyclonal Antibody
cat.: ER62480
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 227 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MYH9 aa 1,151-1,430. |
| Positive control: | A431 cell lysate, HT-29 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, A549 cell lysate, Mouse lung tissue lysate, Mouse liver tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, HeLa. |
| Subcellular location: | Cytoplasm, cytoskeleton, cell cortex, Cytoplasmic vesicle, secretory vesicle, Cortical granule, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:10,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P35579 Human | Q8VDD5 Mouse | Q62812 Rat |
| Alternative names: | BDPLT 6 BDPLT6 Cellular myosin heavy chain Cellular myosin heavy chain type A DFNA 17 DFNA17 EPSTS FTNS MGC104539 MHA MYH 2A MYH 9 MYH2A MYH9 MYH9_HUMAN MYHas8 MyHC 2A MyHC IIa MyHC2A MyHCIIa MYHSA 2 MYHSA2 Myosin 9 Myosin heavy chain 9 Myosin heavy chain 9 non muscle Myosin heavy chain Myosin heavy chain non muscle IIa Myosin heavy chain nonmuscle IIa Myosin heavy polypeptide 2 Myosin heavy polypeptide 9 non muscle Myosin-9 Myosin9 NMHC II A NMMHC A NMMHC II a NMMHC II-a NMMHC IIA NMMHC-A NMMHC-IIA NMMHCA Non muscle myosin heavy chain A Non muscle myosin heavy chain Non muscle myosin heavy chain II A Non muscle myosin heavy polypeptide 9 non-muscle IIa Non-muscle myosin heavy chain A Non-muscle myosin heavy chain IIa Nonmuscle myosin heavy chain A Nonmuscle myosin heavy chain II A type A |
Images
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Fig1:
Western blot analysis of non-muscle Myosin IIA on different lysates with Rabbit anti-non-muscle Myosin IIA antibody (ER62480) at 1/10,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: Mouse lung tissue lysate (40 µg/Lane) Lane 8: Mouse liver tissue lysate (40 µg/Lane) Lane 9: Rat kidney tissue lysate (40 µg/Lane) Lane 10: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 227 kDa Observed band size: 227 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER62480) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling non-muscle Myosin IIA with Rabbit anti-non-muscle Myosin IIA antibody (ER62480) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-non-muscle Myosin IIA antibody (ER62480) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling non-muscle Myosin IIA. Cells were fixed and permeabilized. Then stained with the primary antibody (ER62480, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.