alpha 2a Adrenergic Receptor Rabbit Polyclonal Antibody
cat.: ER62703
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ADRA2A aa 1-48.
Positive control: SH-SY5Y cell lysate, PC-12 cell lysate, Mouse hippocampus tissue lysate, Mouse brain tissue lysate, Rat hippocampus tissue lysate, Rat brain tissue lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:10,000
Uniprot #: SwissProt: P08913 Human
Alternative names: ADA2A_HUMAN ADRA2 ADRA2A ADRA2R ADRAR Adrenergic alpha 2A receptor Alpha 2 adrenergic receptor subtype C10 Alpha 2A adrenergic receptor Alpha 2AAR subtype C10 Alpha-2 adrenergic receptor subtype C10 alpha-2-adrenergic receptor platelet type Alpha-2A adrenergic receptor Alpha-2A adrenoceptor Alpha-2A adrenoreceptor Alpha-2AAR ALPHA2AAR OTTHUMP00000058849 ZNF32 AR α2A ARα2A
Images
ER62703_1.jpg Fig1: Western blot analysis of alpha 2a Adrenergic Receptor on different lysates with Rabbit anti-alpha 2a Adrenergic Receptor antibody (ER62703) at 1/10,000 dilution.

Lane 1: SH-SY5Y cell lysate (no heat)
Lane 2: PC-12 cell lysate
Lane 3: Mouse hippocampus tissue lysate (no heat)
Lane 4: Mouse brain tissue lysate (no heat)
Lane 5: Rat hippocampus tissue lysate
Lane 6: Rat brain tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 15 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER62703) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.