DNAJC5 Rabbit Polyclonal Antibody
cat.: ER63138
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey, Pig |
| Applications: | WB, IF-Cell, FC(Intra) |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DNAJC5 aa 151-198. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, Hep G2 (Human liver cancer cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse brain (P7) tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane , Melanosome, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC(Intra) |
1:2,000 1:100 1:200 |
| Uniprot #: | SwissProt: Q9H3Z4 Human | P60904 Mouse | P60905 Rat |
| Alternative names: | CLN 4 CLN4 CLN4B CSP cysteine string protein alpha Cysteine string protein DJC5 DnaJ (Hsp40) homolog subfamily C member 5 DnaJ homolog subfamily C member 5 DNAJC 5 Dnajc5 DNAJC5A DNJC5_HUMAN NCL |
Images
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Fig1:
Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (ER63138) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 3: Hep G2 (Human liver cancer cell) cell lysate Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate Lane 5: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 7: C6 (Rat glioma cell) cell lysate Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER63138, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 30 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell) Species: Rat Sample: PC-12(Rat pheochromocytoma cell (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: ER63138, 1/100, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (ER63138) at 1/2,000 dilution. Lane 1: Mouse brain (P7) tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate Lane 5: Pig brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 14 seconds; ECL: K1801 Negative expression of DNAJC5 protein in kidney is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER63138, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 22 kDa Observed band size: 30 kDa |
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Fig4:
Application: Flow Cytometry (Intra) Species: Rat Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: ER63138(1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.