EGR3 Rabbit Polyclonal Antibody
cat.: ER63324
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human EGR3 aa 171-220. |
| Positive control: | MCF7 cell lysate, PC-3 cell lysate, LNCaP cell lysate, Mouse colon tissue lysate, Mouse kidney tissue lysate, Rat colon tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q06889 Human | P43300 Mouse | P43301 Rat |
| Alternative names: | Early growth response 3 Early growth response gene 3 Early growth response protein 3 Early growth responsive 3 EGR 3 MGC138484 Pilot Zinc finger protein pilot |
Images
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Fig1:
Western blot analysis of EGR3 on different lysates with Rabbit anti-EGR3 antibody (ER63324) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: PC-3 cell lysate Lane 3: LNCaP cell lysate Lane 4: Mouse colon tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 43 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63324) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.