Hexokinase Type III / HK3 Rabbit Polyclonal Antibody
cat.: ER63723
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 100 kDa
Isotype: IgG
Positive control: Mouse spleen tissue lysate, Mouse colon tissue lysate.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q3TRM8 Mouse
Alternative names: ATP:D hexose 6 phosphotransferase Hexokinase type III Hexokinase-3 Hexokinase3 HK III Hk3 HKIII HXK3 HXK3_HUMAN
Images
ER63723_1.jpg Fig1: Western blot analysis of Hexokinase Type III / HK3 on different lysates with Rabbit anti-Hexokinase Type III / HK3 antibody (ER63723) at 1/1,000 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse colon tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63723) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.