| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Positive control: | Mouse spleen tissue lysate, Mouse colon tissue lysate. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q3TRM8 Mouse |
| Alternative names: | ATP:D hexose 6 phosphotransferase Hexokinase type III Hexokinase-3 Hexokinase3 HK III Hk3 HKIII HXK3 HXK3_HUMAN |
|
Fig1:
Western blot analysis of Hexokinase Type III / HK3 on different lysates with Rabbit anti-Hexokinase Type III / HK3 antibody (ER63723) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse colon tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63723) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |