IFNGR1 Rabbit Polyclonal Antibody
cat.: ER63732
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IFNGR1 aa 18-245 (Extracellular). |
| Positive control: | NCI-H226 cell lysate, LoVo cell lysate, LoVo. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:3,000 1:1,000 |
| Uniprot #: | SwissProt: P15260 Human |
| Alternative names: | Antiviral Protein Type II Antiviral protein, type 2 AVP type II AVP, type 2 CD 119 CD119 CD119 antigen CDw119 FLJ45734 IFN gamma R alpha IFN gamma R IFN gamma R1 IFN-gamma receptor 1 IFN-gamma-R1 IFNG R1 IFNGR 1 IFNGR IFNGR1 Immune interferon receptor 1 Immune interferon receptor for INGR1_HUMAN Interferon gamma receptor 1 Interferon gamma receptor alpha chain Interferon gamma receptor alpha chain precursor |
Images
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Fig1:
Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (ER63732) at 1/2,000 dilution. Lane 1: NCI-H226 cell lysate (20 µg/Lane) Lane 2: LoVo cell lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 70-100 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63732) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (ER63732) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IFNGR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63732) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody (ER63732) at 1/3,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody (ER63732) at 1/3,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of LoVo cells labeling IFNGR1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER63732, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.