MRG15 Rabbit Polyclonal Antibody
cat.: ER64000
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MRG15 aa 1-150. |
| Positive control: | Huh7 cell lysate, HeLa cell lysate, C2C12 cell lysate, HeLa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: Q9UBU8 Human | P60762 Mouse |
| Alternative names: | CG6363 Eaf3 Esa1p associated factor 3 homolog FWP006 HsT17725 MEAF3 MO4L1_HUMAN MORF related gene on chromosome 15 MORF-related gene 15 protein Morf4l1 MORFRG15 Mortality factor 4 like 1 Mortality factor 4 like protein 1 Mortality factor 4-like protein 1 MRG15 Protein MSL3-1 S863-6 Transcription factor-like protein MRG15 |
Images
|
Fig1:
Western blot analysis of MRG15 on different lysates with Rabbit anti-MRG15 antibody (ER64000) at 1/2,000 dilution. Lane 1: Huh7 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute 39 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64000) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling MRG15 with Rabbit anti-MRG15 antibody (ER64000) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MRG15 antibody (ER64000) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.