MYPT1 Rabbit Polyclonal Antibody
cat.: ER64090
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 115 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MYPT1 aa 681-730. |
| Positive control: | 293T cell lysate, HeLa cell lysate, C2C12 cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, stress fiber. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: O14974 Human | Q9DBR7 Mouse | Q10728 Rat |
| Alternative names: | M130 MBS Myosin binding subunit Myosin phosphatase target subunit 1 Myosin phosphatase targeting subunit 1 Myosin phosphatase-targeting subunit 1 MYPT1 MYPT1_HUMAN PPP1R12A Protein phosphatase 1 regulatory inhibitor subunit 12A Protein phosphatase 1 regulatory subunit 12A Protein phosphatase 1, regulatory (inhibitor) subunit 12A Protein phosphatase myosin binding subunit Protein phosphatase myosin-binding subunit |
Images
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Fig1:
Western blot analysis of MYPT1 on different lysates with Rabbit anti-MYPT1 antibody (ER64090) at 1/10,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 140 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64090) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.