NDUFS1 Rabbit Polyclonal Antibody
cat.: ER64138
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDUFS1 aa 201-727. |
| Positive control: | HepG2 cell lysate, Caco-2 cell lysate, HeLa cell lysate, C2C12 cell lysate, L6 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: P28331 Human | Q91VD9 Mouse | Q66HF1 Rat |
| Alternative names: | CI-75kD Complex I 75Kd Complex I, mitochondrial respiratory chain, 75 kD subunit Complex I-75kD mitochondrial NADH coenzyme Q reductase NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit NDUFS1 NDUS1_HUMAN PRO1304 |
Images
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Fig1:
Western blot analysis of NDUFS1 on different lysates with Rabbit anti-NDUFS1 antibody (ER64138) at 1/10,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Caco-2 cell lysate Lane 3: HeLa cell lysate Lane 4: C2C12 cell lysate Lane 5: L6 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64138) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.