Oxytocin-neurophysin 1 / OXT Rabbit Polyclonal Antibody
cat.: ER64173
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OXT aa 1-125. |
| Positive control: | Mouse pituitary tissue lysates, mouse pituitary tissue, rat pituitary tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:8,000 |
| Uniprot #: | SwissProt: P35454 Mouse Entrez Gene: 25504 Rat |
| Alternative names: | MGC126890 MGC126892 Neurophysin 1 Neurophysin I Ocytocin OT OT NPI OXT Oxytocin Oxytocin Neurophysin preproprotein Oxytocin prepro (neurophysin I) Oxytocin prepropeptide Oxytocin-neurophysin 1 |
Images
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Fig1:
Western blot analysis of Oxytocin-neurophysin 1 / OXT on mouse pituitary tissue lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (ER64173) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 13 kDa Observed band size: 14 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64173) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (ER64173) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER64173) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (ER64173) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER64173) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.