PERK Rabbit Polyclonal Antibody
cat.: ER64553
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 125 kDa |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996 |
| Positive control: | MCF-7 cell, human colon carcinoma, rat-spleen. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-cell IHC-P |
1:500-1:2,000 1:50-200 1:100-1:300 |
| Uniprot #: | SwissProt: Q9NZJ5 Human |
| Alternative names: | DKFZp781H1925 E2AK3_HUMAN EC 2.7.11.1 Eif2ak3 Eukaryotic translation initiation factor 2 alpha kinase 3 Eukaryotic translation initiation factor 2-alpha kinase 3 Heme regulated EIF2 alpha kinase HRI HsPEK Pancreatic eIF2 alpha kinase Pancreatic eIF2-alpha kinase PEK PRKR like endoplasmic reticulum kinase PRKR-like endoplasmic reticulum kinase WRS |
Images
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Fig1: Western blot analysis of lysates from MCF-7 cells, using PEK/PERK Antibody. The lane on the right is blocked with the synthesized peptide. |
|
Fig2: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PEK/PERK Antibody. The picture on the right is blocked with the synthesized peptide. |
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Fig3: Immunofluorescence analysis of rat-spleen tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.