PJA2 Rabbit Polyclonal Antibody
cat.: ER64613
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 78 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PJA2 aa 31-80.
Positive control: HeLa cell lysate, Jurkat cell lysate, U-87 MG cell lysate.
Subcellular location: Cytoplasm, Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Synapse, Postsynaptic density, cytoskeleton, microtubule organizing center, centrosome.
Recommended Dilutions:
  WB
1:1,000
Uniprot #: SwissProt: O43164 Human
Alternative names: E3 ubiquitin protein ligase Praja2 E3 ubiquitin-protein ligase Praja-2 KIAA0438 Neurodap 1 Neurodap1 PJA 2 PJA2 PJA2_HUMAN Praja 2 Praja 2 RING H2 motif containing praja ring finger 2 Praja ring finger 2 E3 ubiquitin protein ligase Praja2 RING finger protein 131 RNF 131 RNF131
Images
ER64613_1.jpg Fig1: Western blot analysis of PJA2 on different lysates with Rabbit anti-PJA2 antibody (ER64613) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: U-87 MG cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 78 kDa
Observed band size: 150 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64613) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.