| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SRPK1 aa 251-500. |
| Positive control: | Jurkat cell lysate, Caco-2 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, BxPC-3 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, nucleoplasm, Nucleus speckle, Chromosome. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: Q96SB4 Human |
| Alternative names: | Serine/arginine rich protein specific kinase 1 Serine/arginine rich splicing factor kinase 1 Serine/arginine-rich protein-specific kinase 1 Serine/threonine protein kinase SRPK1 Serine/threonine-protein kinase SRPK1 SFRS protein kinase 1 SFRSK1 SR protein kinase 1 SR protein specific kinase 1 SR-protein-specific kinase 1 SRPK1 SRPK1_HUMAN |
|
Fig1:
Western blot analysis of SRPK1 on different lysates with Rabbit anti-SRPK1 antibody (ER65028) at 1/10,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Caco-2 cell lysate Lane 3: HeLa cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: BxPC-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 100 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65028) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |