SRPK1 Rabbit Polyclonal Antibody
cat.: ER65028
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 74 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SRPK1 aa 251-500.
Positive control: Jurkat cell lysate, Caco-2 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, BxPC-3 cell lysate.
Subcellular location: Cytoplasm, Nucleus, nucleoplasm, Nucleus speckle, Chromosome.
Recommended Dilutions:
  WB

1:10,000
Uniprot #: SwissProt: Q96SB4 Human
Alternative names: Serine/arginine rich protein specific kinase 1 Serine/arginine rich splicing factor kinase 1 Serine/arginine-rich protein-specific kinase 1 Serine/threonine protein kinase SRPK1 Serine/threonine-protein kinase SRPK1 SFRS protein kinase 1 SFRSK1 SR protein kinase 1 SR protein specific kinase 1 SR-protein-specific kinase 1 SRPK1 SRPK1_HUMAN
Images
ER65028_1.jpg Fig1: Western blot analysis of SRPK1 on different lysates with Rabbit anti-SRPK1 antibody (ER65028) at 1/10,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: BxPC-3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 74 kDa
Observed band size: 100 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65028) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.