TNF alpha Rabbit Polyclonal Antibody
cat.: ER65189
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 16 kDa |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190 |
| Positive control: | Blood,Peripheral blood leukocyte,Placenta,Prostatic carcinoma, |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-cell |
1:500-1:2000 1:100-1:300 1:50-1:200 |
| Uniprot #: | SwissProt: P01375 Human |
| Alternative names: | APC1 APC1 protein Cachectin DIF Differentiation inducing factor Macrophage cytotoxic factor Tnf TNF superfamily member 2 TNF superfamily member 2 TNF macrophage derived TNF monocyte derived TNF-a TNF-alpha TNFA TNFA_HUMAN TNFSF2 Tumor necrosis factor (TNF superfamily member 2) Tumor necrosis factor alpha Tumor necrosis factor Tumor necrosis factor ligand superfamily member 2 Tumor Necrosis Factor Membrane Form Tumor necrosis factor soluble form TNF-α TNF α |
Images
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Fig1: Western Blot analysis of jurkat cell lysate using TNF-α Polyclonal Antibody. Secondary antibody(catalog#:HA1001) was diluted at 1:20000. |
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Fig2: Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Fig3: Immunofluorescence analysis of human-stomach tissue. 1,TNF-α Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.