ULK3 Rabbit Polyclonal Antibody
cat.: ER65265
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ULK3 aa 1-472 / 472. |
| Positive control: | 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q6PHR2 Human | Q3U3Q1 Mouse | D3ZHP7 Rat |
| Alternative names: | DKFZP434C131 FLJ90566 Serine/threonine-protein kinase ULK3 Ulk3 ULK3_HUMAN unc 51 like kinase 3 (C. elegans) Unc-51-like kinase 3 |
Images
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Fig1:
Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (ER65265) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65265) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling ULK3. Cells were fixed and permeabilized. Then stained with the primary antibody (ER65265, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.